News and Events | Membrane Protein Structural Dynamics Gateway

2019 Biological CryoEM workshop video and photos released

Posted on July 3, 2019 in Workshops

Thanks to all for attending the 2019 Workshop on Advancement and Challenges of Biological cryoEM in Hong Kong.

Workshop photos

http://memprotein.org/cryoem-workshop/workshop-photos/

Video, session 3: Discussion on building a Sustainable Cryo-EM Community

Chair:

Yifan Cheng, University of California, San Diego

Panelists:

Lu Gan, National University of Singapore
Peiyi Wang, Southern University of Science and Technology
Yong Zi Tan, Columbia University / New York Structural Biology Center
Alex Noble, New York Structutral Biology Center

2019 Workshop on Advances and Challenges of Biological CryoEM

Posted on March 22, 2019 in Workshops

(High resolution video can be accessed here: https://cryoem101.org/chapter-1/)

The recent integration of new developments in electron microscopes, direct electron detection cameras, and advances in image analysis methods are allowing the expansion of high resolution structural molecular biology in new and exciting directions by direct visualization of macromolecules and their complexes. The next decades will be dominated by the study of protein-protein, protein-nucleic acid complexes, molecular machines, and their conformational changes in ways that were impossible before due to their size and/or the need to study them in crystalline form. In addition, developments in many aspects of electron microscopy are providing new tools for the study of biological molecules from the single molecule to the cellular level. The inaugural workshop on Advancement and Challenges of Biological CryoEM will address key developments in this fast advancing field, provide an opportunity to discuss the state of the field and exchange views on advancement and challenges of biological cryo-electron microscopy, and foster the research collaboration of biological and biomedical sciences, particularly in Hong Kong, China, and Asia. The workshop will be hold the day after the GRC Conference on three-dimension electron microscopy in Hong Kong, which is the premier cryoEM meeting, to encourage the participation of GRC attendees.

Visit the website »

Third Coast Workshop on Biological Cryo-EM announced for May 19, 2018

Posted on March 9, 2018 in Upcoming Events

March 19, 2018 • 9:00am – 6:00pm
Gordon Center for Integrative Science, University of Chicago

Friends,

As announced a few months ago, a new version of our very successful Third Coast cryo-EM Workshop is being programmed for May 19th at the Gordon Center for Integrative Sciences at the University of Chicago.

Following last year format, we will have a full day of talks and discussions led by key luminaries in the application of cryo electron microscopy and associated computational approaches to the structure of macromolecules, macromolecular complexes and subcellular structures. In this version of the program (included below) we are privileged to have Prof. Michael Rossmann (Purdue University) as this year’s keynote lecturer.

The website is now live at http://memprotein.org/cryoem-workshop and includes a registration link. There is no cost to attend the Workshop. However, this year, we will close the registration process as soon as the capacity of the auditorium is reached. For that reason, we strongly encourage you to register early, given the unexpected (overwhelming!) response last year. Furthermore, we strongly encourage all attendants to submit posters for discussion during the Workshop. This is a fantastic opportunity to show your work to a receptive community, whether it is ready for prime time, or not.

Given the stellar roster of speakers, we expect to have an exciting workshop where the key cryo-EM topics of the moment will be presented and debated. Personally, we are very much looking forward to seeing you and interacting with you later in May.

All the best,

The Organizing Committee

Eduardo Perozo
Alfonso Mondragon
Valerie Tokars
Bobby Kasthuri

Program, registration, and poster at the website: http://memprotein.org/cryoem-workshop/

Final Program for III Frontiers in Membrane Protein Structural Dynamics Announced

Posted on November 8, 2017 in Upcoming Events

Friends,

As we get everything in order to welcome you this Friday to our Frontiers in Membrane Proteins Structural Dynamics Conference, we wanted to share with you the final program of the meeting.

The next few days promise to be a truly exceptional survey of membrane proteins structure and dynamics today. A veritable state of the art of the field. With our two keynote speakers (Chris Miller and Olga Boudker) leading 8 individual mini-symposia.

Personally, we are very much looking forward to seeing you and interacting with you later this week.

All the best,

The Organizing Committee

III Frontiers in membrane Protein Structural Dynamics
APS Conference Center, Argonne National Lab

Friday, November 10

7:45	Continental Breakfast, APS 402 Atrium
8:15	Eduardo Perozo (University of Chicago), Stephen Streiffer (Advanced Photon Source) Welcome and overall perspective

Session I: ABC Transporters and Partners: Dynamics at the Edge of Stability

8:25	Christine Ziegler (Universität Regensburg) Introduction
8:30	Doug Rees (Caltech University) ABC transporters and the alt-access movement
8:55	Show-Ling Shyng (Oregon Health & Science University) Illumination of the SUR1-Kir6.2 partnership in KATP channels by single-particle cryo-EM
9:20	Heather Pinkett (Northwestern University) The role of ABC transporters in nutrient uptake and pathogenesis

9:45	Break, APS 402 Atrium

Session II: Technology Advances in Protein Expression and Engineering

10:25	Chris Ahern (University of Iowa) Introduction
10:30	Anthony Kossiakoff (University of Chicago) Synthetic Antibodies to facilitate structural analyses of functional conformational intermediates by both crystallography and Cryo-EM
10:55	Bill Clemons (Caltech University) Solving the membrane protein expression problem
11:20	Andrew Kruse (Harvard University) New technologies to interrogate membrane protein function

11:45

Lunch, APS 402 – Lower Gallery

Session III: Pumping Ions and Other Flotsam

1:00	Francisco Bezanilla (University of Chicago) Introduction
1:05	Bernd Fakler (University of Freiburg, Germany) Native PMCAs – proteomic surprises with old Ca2+-pumps
1:30	Miguel Holmgren (National Institutes of Health) Dynamics of the Na/K ATPase with the binding/unbinding of external Na ions
1:55	Huan Rui (University of Chicago) Probing the cycling mechanism of the P-type ATPase: Insights from kinetic modeling simulations

2:20	Break, APS 402 Atrium

Session IV: Poster Rapid Fire

2:50	Yeong-Kyun Shin (Iowa State University) Introduction
2:55	Poster presenters 2 minute talks

Session V: Keynote Speaker 1

4:45	A Brief Vignette Honoring Jean Chin (Eduardo Perozo, University of Chicago)
5:00	Alessio Accardi (Weill Cornell University) Introduction
5:10	Christopher Miller (Brandeis University) How channel crystallography made me stupid

6:00	Dinner, APS 402 – Lower Gallery Posters, APS 402 – E1100/E1200

Saturday, November 11

Session VI: Calculating Conformational Changes: At the Bleeding Edge

7:45	Continental Breakfast, APS 402 Atrium
8:25	Wonpil Im (LeHigh University) Introduction
8:30	Benoit Roux (University of Chicago) C-type Inactivation and the Constricted-Like Conformations of the Selectivity Filter of K+ Channels
8:55	Michael Grabe (University of California, San Francisco) TMEM16 lipid scrambles bend membranes to get things done
9:20	Jose Faraldo-Gomez (National Institutes of Health) The Dos and Don’ts of the Alternating-Access Mechanism: Lessons from the Sodium-Calcium Exchanger

9:45	Break, APS 402 Atrium

Session VII: Protein Motion Within a Field: Ins and Outs of Voltage-dependent Gating

10:25	Sudha Chakrapani (Case Western Reserve University) Introduction
10:30	Jian Yang (Columbia University) Cryo-EM structure of a eukaryotic cyclic nucleotide-gated channel
10:55	Bonnie Wallace (Birbeck, University of London) Structural, Function and Disease-Related Aspects of Sodium Channel Voltage-Gating
11:20	Francisco Bezanilla (University of Chicago) Voltage sensors and membrane capacitance
11:45	Rama Ranganthan (University of Chicago) Protein mechanics: the link between structure, function, and evolution

12:10

Lunch, APS 402 – Lower Gallery

Session VIII: Structure and Dynamics with Few Molecules (or a Lot)

1:25	Valeria Vasquez (University of Tennessee, Memphis) Introduction
1:30	Robert Fischetti (APS, Argonne National Lab) Serial crystallography with monochromatic and polychromatic X-ray beams, and the APS Upgrade
1:55	Yeon-Kyun Shin (Iowa State University) Zooming in on single vehicle fusion
2:20	Simon Scheuring (Weill Cornell University) High-Speed Atomic Force Microscopy: A New Tool for the Study of the Dynamics of Single Unlabeled Membrane Proteins

2:45	Break, APS 402 Atrium Posters, APS 401 – E1100/E1200

Session IX: The Good, the Bad and the Ugly: Conformational Changes and the Transport/Translocation Cycle

4:30	Ming Zhou (Baylor University) Introduction
4:35	Filipo Mancia (Columbia University) The Molecular Mechanisms Underlying Cellular Uptake of Vitamin A
5:00	Aurelio Galli (University of Vanderbilt) Failure to Prime the Dopamine ‘Pump’ in Autism
5:25	Alessio Accardi (Weill Cornell University) Exploring the backdoor of CLC channels and transporters: how a glutamate gets in and out of the Cl- permeation pathway

5:50	Dinner, APS 402 – Lower Gallery Posters, APS 401 – E1100/E1200

Sunday, November 12

Session X: Structural Dynamics Through Imaging

9:00	Tobin Sosnick (University of Chicago) Introduction
9:05	Christine Ziegler (Universität Regensburg) Being in the right place: Localization-dependent lipid interactions in the PC2 TRP Channel
9:30	Irina Serysheva (University of Texas Houston) Structure of IP3R Channel: Towards Understanding Gating Mechanism
9:55	Vera Moiseenkova-Bell (University of Pennsylvania) Molecular mechanism of the TRPV2 channel pore dynamics during ligand activation
10:20	Mingley Zhao (University of Chicago) Molecular Mechanism of SNARE Complex Disassembly

10:55

Break, APS 402 – Atrium

Session XI: Keynote Speaker 2

11:05	Robert Nakamoto (University of Virginia) Introduction
11:15	Olga Boudker (Weill Cornell University) Dynamic underpinnings of transport mechanism in glutamate transporters

Closing

*Shuttle service from the guest house to the conference center will start 15 minutes prior to the start of the meeting.

*Shuttle service from the conference center to the guest house will run for 15 minutes after the poster session (9:00pm).

Announcing Frontiers in Membrane Protein Structural Dynamics 2017

Posted on June 23, 2017 in Workshops

Every other year, as part of our NIH sponsored Membrane Protein Structural Dynamics Consortium we gather in Chicago for a conference to discuss the most exciting aspects at the frontiers of membrane protein structure functional and dynamics. We (and the NIH) see this as gathering of the leaders in the field and a great opportunity to interface the advances of the Consortium with the community at large.

We are happy to announce the newest version of our very successful “Frontiers in Membrane Protein Structural Dynamics” meeting. It will take place on November 10-12 at the Advanced Photon Source Conference center at Argonne National Lab. We are still finalizing the program but it is expected to have a full slate of very exciting talks. The conference will begin on the evening of Friday, November 10th and end in the afternoon on Sunday, the 12th. There will be a total of 8 symposia sessions, and two keynote talks, bookending the meeting, plus an extensive poster session accompanied by a “flash session” where poster presenters will summarize their work in 1 slide/2 min micro talks.

As before, we hope to encourage attendance of graduate students and post-doctoral fellows by having a low non-speaker participant fee and offering travel awards to students, postdocs, and new faculty.

An on-line registration site will be available soon for this meeting at: http://memprotein.org/meeting

Looking very much forward to have you in the Chicago area in November!

With our best wishes (the organizing committee),

Eduardo Perozo
Hassane Mchaourab
Robert Nakamoto
Robert Fischetti
Olga Boudker
Chris Ahern